Molecular Evolution of the Pathogenicity Island of Enterotoxigenic Bacteroides fragilis Strains
نویسندگان
چکیده
منابع مشابه
Molecular evolution of the pathogenicity island of enterotoxigenic Bacteroides fragilis strains.
Enterotoxigenic Bacteroides fragilis (ETBF) strains, which produce a 20-kDa zinc metalloprotease toxin (BFT), have been associated with diarrheal disease in animals and young children. Studying a collection of ETBF and nontoxigenic B. fragilis (NTBF) strains, we found that bft and a second metalloprotease gene (mpII) are contained in an approximately 6-kb pathogenicity island (termed B. fragili...
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Enterotoxigenic (ETBF) strains of Bacteroides fragilis are the subset of strains that secrete a toxin called fragilysin (Bft). Although ETBF strains are known to cause diarrheal disease and have recently been associated with colorectal cancer, they have not been well characterized. By sequencing the complete genome of four ETBF strains, we found that these strains exhibit considerable variation...
متن کاملMolecular characterization of the fragilysin pathogenicity islet of enterotoxigenic Bacteroides fragilis.
Enterotoxigenic strains of Bacteroides fragilis produce an extracellular metalloprotease toxin (termed fragilysin) which is cytopathic to intestinal epithelial cells and induces fluid secretion and tissue damage in ligated intestinal loops. We report here that the fragilysin gene is contained within a small genetic element termed the fragilysin pathogenicity islet. The pathogenicity islet of B....
متن کاملEnterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil.
The presence of enterotoxigenic Bacteroides fragilis and nontoxigenic B. fragilis (NTBF) among 109 strains isolated from 1980-2008 in Brazil were investigated by PCR. One strain, representing 0.9% of the total analyzed strains, harbored the bft gene which was identified as bft-1 isoform based on PCR-RFLP and sequencing. Forty-nine strains (44.9%) exhibited the NTBF pattern III which possesses t...
متن کاملDetection of enterotoxigenic Bacteroides fragilis by PCR.
Strains of enterotoxigenic Bacteroides fragilis (ETBF) are associated with diarrhea in young farm animals and, at least in particular settings, in children. Enterotoxin production by ETBF is currently detected by a tissue culture assay with HT-29 cells. We have developed a PCR assay based on the detection of the enterotoxin gene to identify ETBF in culture and in stool samples. Overall, 113 bac...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1999
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.181.21.6623-6633.1999